Digital Photographic Practices as Expressions of Personhood and Identity:Variations Across School Leavers and Recent Retirees

Over the last two decades, digital photography has been adopted by young and old. Many young adults easily take photos, share them across multiple social networks using smartphones, and create digital identities for themselves consciously and unconsciously. Is the same true for older adults? As part of a larger mixed-methods study of online life in the UK, we considered digital photographic practices at two life transitions: leaving secondary school and retiring from work. In this paper, we report on a complex picture of different kinds of interactions with visual media online, and variation across age groups in the construction of digital identities. In doing so, we argue for a blurring of the distinctions between Chalfen’s ‘Kodak Culture’ and Miller and Edwards’ ‘Snaprs’. The camera lens often faces inwards for young adults: tagged ‘Selfies’ and images co-constructed with social network members commonly contribute to their digital identities. In contrast, retirees turn the camera’s lens outwards towards the world, not inwards to themselves. In concluding, we pay special attention to the digital social norms of co-creation of self and balancing convenience and privacy for people of varying ages, and what our findings mean for the future of photo-sharing as a form of self-expression, as today’s young adults grow old and retire

Fraping, social norms and online representations of self

Charting the Digital Lifespan research project was funded by EPSRC Grant Reference No EP/L00383X/1.This paper reports on qualitative insights generated from 46 semi-structured interviews with adults ranging in age from 18 to 70. It focuses on an online social behaviour, ‘fraping’, which involves the unauthorised alteration of content on a person's social networking site (SNS) profile by a third party. Our exploratory research elucidates what constitutes a frape, who is involved in it, and what the social norms surrounding the activity are. We provide insights into how frape contributes to online sociality and the co-construction of online identity, and identify opportunities for further work in understanding the interplay between online social identities, social groups and social norms.PostprintPeer reviewe

Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- And Rab8-Dependent and recycling endosome-independent

The intermediate conductance, Ca2+-activated K+ channel (KCa3.1) targets to the basolateral (BL) membrane in polarized epithelia where it plays a key role in transepithelial ion transport. However, there are no studies defining the anterograde and retrograde trafficking of KCa3.1 in polarized epithelia. Herein, we utilize Biotin Ligase Acceptor Peptide (BLAP)-tagged KCa3.1 to address these trafficking steps in polarized epithelia, using MDCK, Caco-2 and FRT cells. We demonstrate that KCa3.1 is exclusively targeted to the BL membrane in these cells when grown on filter supports. Following endocytosis, KCa3.1 degradation is prevented by inhibition of lysosomal/proteosomal pathways. Further, the ubiquitylation of KCa3.1 is increased following endocytosis from the BL membrane and PR-619, a deubiquitylase inhibitor, prevents degradation, indicating KCa3.1 is targeted for degradation by ubiquitylation. We demonstrate that KCa3.1 is targeted to the BL membrane in polarized LLC-PK1 cells which lack the m1B subunit of the AP-1 complex, indicating BL targeting of KCa3.1 is independent of μ1B. As Rabs 1, 2, 6 and 8 play roles in ER/Golgi exit and trafficking of proteins to the BL membrane, we evaluated the role of these Rabs in the trafficking of KCa3.1. In the presence of dominant negative Rab1 or Rab8, KCa3.1 cell surface expression was significantly reduced, whereas Rabs 2 and 6 had no effect. We also co-immunoprecipitated KCa3.1 with both Rab1 and Rab8. These results suggest these Rabs are necessary for the anterograde trafficking of KCa3.1. Finally, we determined whether KCa3.1 traffics directly to the BL membrane or through recycling endosomes in MDCK cells. For these studies, we used either recycling endosome ablation or dominant negative RME-1 constructs and determined that KCa3.1 is trafficked directly to the BL membrane rather than via recycling endosomes. These results are the first to describe the anterograde and retrograde trafficking of KCa3.1 in polarized epithelia cells. © 2014 Bertuccio et al